Here are some protocols that we use in our lab that you can download as PDFs and print. At present, the following items are available:
General protocols. Single document including DNA extraction, PCR, PCR purification, DNA sequencing, etc.
Fungal DNA mini-prep using the E.Z.N.A. kit.
Forensic DNA mini-prep using the E.Z.N.A. kit. We have found this to be a very effective method for small samples.
High Quality DNA maxi-prep for single copy, protein coding genes and other template challenges.
Cloning protocols using the Topo-TA (fast and high efficiency) and Classic-TA (good and affordable) kits.
Generating competent cells for cloning.
Primer protocols in html or pdf.
New: RPB2 primer sequences and map html or pdf.
More protocols will be posted later.