the hibbett lab

at Clark University

Lab Protocols

Here are some protocols that we use in our lab that you can download as PDFs and print. At present, the following items are available:

General protocols. Single document including DNA extraction, PCR, PCR purification, DNA sequencing, etc.

Fungal DNA mini-prep using the E.Z.N.A. kit.

Forensic DNA mini-prep using the E.Z.N.A. kit. We have found this to be a very effective method for small samples.

High Quality DNA maxi-prep for single copy, protein coding genes and other template challenges.

Cloning protocols using the Topo-TA (fast and high efficiency) and Classic-TA (good and affordable) kits.

Generating competent cells for cloning.

Primer protocols in html or pdf.

New: RPB2 primer sequences and map html or pdf.

More protocols will be posted later.