Results of the 2005 Labor Day Mosquito Count

Many thanks for participating in the 2005 Labor Day Mosquito Count!  We appreciate your patience as we report back to you, and thank you for all your efforts of last fall.  We hope you'll be able to participate again next year, as it is important for us to have repeated samples from the same areas over several years.  We also hope to improve the content we're providing each year.

With this summary, we provide:

If any of you have any questions or requests, please let us know by email

We are hoping to expand our sampling network considerably for the coming fall.  If you have colleagues who might be interested, please let them know about this project and encourage them to contact me.

Thanks again!  We'll be in touch as the next mosquito count approaches, and we wish you a pleasant summer until then.

Best regards,

Todd Livdahl




Update from the 2004 Mosquito Count

When we last reported results for 2004, we indicated that Aedes albopictus might have been found in E. Providence, Rhode Island and Loyal, Wisconsin.  We looked into this further to confirm our species identification, and found that this species was not present in either site.


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Highlights for 2005

We seem to be learning different things each year.  The 2004 Mosquito Count revealed the Asian Tiger mosquito Aedes albopictus to be in several places where it had not been seen before.  This year, we have a similar set of discoveries for another Asian introduced species, Ochlerotatus japonicus.  This mosquito was first found in  New Jersey in 1998, and shortly thereafter was found in the New York City area and Connecticut.  It has become common in Southern New England, and the 2005 Mosquito Count indicates that this species has spread westward, as well.  We might have had some japonicus in last years samples and did not realize it, as we used egg measurements to distinguish most of the eggs.  Ochlerotatus japonicus and Ochlerotatus triseriatus (the native treehole mosquito) lay eggs of very similar size and appearance, and we have not yet processed all samples for distinguishing them.  We have only recently found a way to do this by looking at a region of DNA, so we will keep working on this task.  The finding of many O. japonicus in samples has made identification much more complicated.

Other findings:
  • The site with the most mosquitoes appears to be Houston, PA, where the mean density of eggs was 99.5.  This high density was influenced by one trap that contained 1887 eggs, which were probably laid by at least 30 different mosquitoes. Several places tied for the distinction of having the fewest mosquitoes.
  • The site with the most species laying eggs in our traps was  Hazard, KY, which was the only place where all three species have been found.
  • Those who reported habitat type for the different traps contributed to a comparison of the attractiveness of different types of habitat to female egg-laying mosquitoes.  After combining the data for all the sites, traps in forested habitats attracted the most eggs. 
  • In a comparison conducted in Massachusetts last fall, we found a clear positive influence of leaf litter in the traps, so we will continue to send traps with leaf litter added between the plastic and the liner.
Recognition:

We thank the following groups for participating in their third Labor Day Mosquito Count, and for tolerating all of our mistakes of inexperience during each of those years:

Blacksburg VA (Mike Rosenzweig and SEEDS)
Hazard KY (Barbara Weaver)
Forty Fort PA (Hugh Hughes)
Hammond IN (Jeannette Melcic, Frank M. Melcic, Kathy Stump)
Loyal WI (Barb Kingsbury)
Beach Park IL (Marie Gehrig, Judy Mobile)
Dahinda IL (Sharon Farquer)
Junction IL (Mark York)

The following groups have completed their second year of collaboration, and we're glad we still have you: 

Weirton WV (Andrea Anderson)
Oxford NJ (Christine Musa)
Evans WV (David Rader)
Houston PA (Gary Popiolkowski)
New Cumberland WV (Jules Adam, Stephan Smolski)
Riverside/East Providence RI (Mary McCarthy)
Buckhannon WV (Michele Benson)
Beckley WV (Susan Westfall)
Belle WV (Rachelle Marion)
Bridgeport WV (Robin Anglin)
Largo FL (Tracy Springman)
Fayetteville WV (Wayne Yonkelowitz)

We welcome the following groups who have joined us for the first time in 2005, and we look forward to processing your samples again next year:

Carmel IN (Laurie Yesh)
Pendleton IN (Laura Miller)
Crawfordsville IN (Amy Gillan)
Oxford NJ (Christine Musa)
Beachwood OH (Joe Burwell)
Paxton MA (Ted Purcell, Scott Wahlstrom)




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Background on measures of abundance

Various approaches can be used to summarize the abundance of mosquitoes in an area.  The number of mosquito eggs laid within a trap of the sort we use in this study is an example of the use of a relative measure of abundance.  We obtain a number that is useful in comparing different areas or habitats, or for tracking changes through time, but it does not tell us the number of mosquitoes per unit area or volume of habitat, because we don't know how many mosquitoes within the habitat were actually drawn to the trap, and how many were not.  Nonetheless, we can still talk about density of eggs within the traps themselves, so using the trap as our habitat,
the mean density, or average number of eggs per trap gives us a way to describe each site relative to the others. 

The choices made by our students could have a strong influence on mean density.  Mosquitoes use environmental cues to make decisions about how permanent a container of water is likely to be, as well as whether it's likely to have enough food to support her offspring through their development.  A trap in the middle of the parking lot of an apartment complex is not going to attract as many female mosquitoes looking for good habitats for their offspring as a trap set in a damp, dark forest.  So, locations of traps can affect mean density.

A way to avoid this possible problem is to let the mosquitoes determine which habitats are suitable, and to try to measure the degree of crowding that the mosquitoes experience.  If we count, for each individual, the number of neighbors they share their habitat with, and average this for all the individuals, then our measure is not affected by traps that are laid in places that mosquitoes cannot reach or that they find unacceptable.  This measure of Mean Crowding may actually provide a better description of the conditions experienced by the typical developing mosquito larva, and it is at the larval stage that mosquitoes are most likely to experience competition for food.  We provide sample calculations for mean density and mean crowding here







Raw Data

We will be distributing raw data files to all participating group leaders in the form of Excel files attached to e-mail messages.  For the present, If you wish to see the data, you will need to join our sampling network.  At some later time we plan to make all data completely accessible.

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Maps of sampling sites, and graphic results summaries

mcgroups.html
mc05density.html
mc05crowding.html
Locations of all groups that have participated in the Labor Day Mosquito Count, 2003-2005 Average density of all mosquito species for the 2005 Labor Day Mosquito Count.  Not shown:  Largo, FL and Vega, TX  (density =0), to permit more detail
Mean crowding of all mosquito species for the Labor Day Mosquito Count, 2005.

mc05habitat.html
mc05summary.html
Average density of mosquito eggs in traps set in different habitats, Labor Day Mosquito Count, 2005 Summary of findings by mosquito species, Labor Day Mosquito Count, 2005.  Sites with no eggs laid are not shown.


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Practical difficulties, and potential solutions

During our first three years, we have tried a variety of approaches, including trap design, length of sampling period, and number of samples per group.  It is time to settle on a standard method, and consistency remains a challenge.  We thought we had an improved design over previous years, but the new design turned out to have its own problems, especially drying and tipping of the traps.  When traps dry out, they blow around, and mosquitoes don't recognized them as good places to lay eggs.  Dry traps are uninformative or misleading about how many mosquitoes there are.  This can be avoided by having students add water every three days.  Some means of protection against tipping will also be useful in our next trial.

Some groups did not collect any eggs in their traps.  This is not necessarily a problem, because it is possible that mosquitoes were not present during the sampling period.  In some places, such as El Paso, Texas, it might even be expected.  We might also expect an absence of mosquitoes during dry spells, or after an insecticide spraying program.  However, we wonder if students received uniform advice from teachers about trap location.  This may be a flaw in the study design that we might not be able to overcome, but we will work to illustrate and explain more clearly next fall.

Retaining participants is a continuing challenge.  We thought we might have made the methods too demanding in 2004, so we shortened the collection period to two weeks from three last fall, and this may have contributed to a higher participation rate for 2005.  However, our number of participants has declined and we must make this a priority effort for this summer.  If you can help us to spread the word about this project, we'd appreciate it.



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Overview, and plans for next year

In the first three years of this study, we have expanded knowledge about where two introduced species are living, and this kind of information about past success is useful for predicting where they might succeed or fail in the future.  Our broader goal is to see what the effect of the invading species are on the native mosquito, Ochlerotatus triseriatus.  The project began with a focus on just the Asian species, Aedes albopictus, but we will be expanding our goals to include the influence of Ochlerotatus japonicus, which arrived in the Northeast in the late 1990s.  We saw this species for the first time last fall in our study site in Worcester, MA, and by taking a close look at one place we might be able to detect its effect on the native species, which we have been studying for quite a while prior to the arrival of the invader.

During the summer, we plan to add substantially to the educational content of this site, using materials produced by some of our students during the past year.  We now have access to facilities to produce more interesting video as well as videoconferencing, so we'll try to learn enough to stage a web conference for next fall.  We can't promise this yet, since we don't really know what this will entail, but we will be looking into it.

Thanks again for all your help, and please let us know what we can do to improve this experience for your kids.

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