The Larochelle Lab
at Clark University





Protocols

Fixing Cells

1. Let cells attach to coverslips and remove media.

2. Replace with LoFlo for 1-5 hours if background fluorescence is a problem.

3. Remove media.

4. Fix at -20°C for minutes in prechilled MeOH+1% formaldehyde.

5. Wash 5 minutes in 1X PBS.

General Recipes

10X PBS: for 1L
80 g NaCl
2 g Kcl
14.4 g Na2HPO4
2.4 g KH2PO4
in 800 mL dH2O
pH to 7.4 with HCl and autoclave

50X TAE: for 1L
242 g Tris Base
57.1 mL glacial acetic acid
100 mL of 0.5 M EDTA, pH 8.0

20X SSC:
For 1L
175.3 g NaCl
88.2g Sodium Citrate.2H2O
pH to 7

TE:
10 mM Tris, pH 8
1 mM EDTA




Links | About Prof Larochelle
Clark Biology Dept | Clark University


Worcester, MA | 508.793.7631 | dlarochelle@clarku.edu