The Larochelle Lab
at Clark University

Lab Protocols


DAPI Solution:
2.5 uL DAPI stock
0.07 g NH4Cl
---to 25 mL with 1X PBS

.....After cells have been fixed, add 2 mL of DAPI solution to coverslips in 6 well plates and incubate 10 min at RT in the dark. Wash slides briefly with 1X PBS and then wash for two times for 20-30 minutes.
Mount coverslips onto slides using glycerol +100 mg/mL DABCO (stored at 4°C).The addition of DABCO helps to prevent the fading of fluorescence. Store slides at 4°C in the dark.



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