The Larochelle Lab
at Clark University





PROTEIN SAMPLES

10X Wash Buffer (for protein samples):

            0.1 mM MES pH 6.8
            2.5 mM EGTA
            1 mM MgCl2

Lysis Buffer: (for 20 mL)

            2 mL 10X Wash Buffer (for protein samples)
            10 uL of 20% Triton X-100
            2 uL of 5 mg/mL Leupeptin
            20 uL of 0.1 M PMSF (in 100% EtOH-stored at 4˚C)
            2 uL of 1.4 mg/mL pepstatinA

2X Sample Buffer:  For 10 mL

            0.12 g ..... ..... .....100 mM Tris Base 
            0.31 g ..... ..... .....200 mM DTT 
            0.40 g ..... ..... .....4% SDS  
            tip of small spatula 0.2% Bromophenol Blue 
            2 mL of 100%. ..... 20% glycerol ..... .






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Clark Biology Dept | Clark University



Worcester, MA | 508.793.7631 | dlarochelle@clarku.edu