PROTEIN SAMPLES
10X Wash Buffer (for protein samples):
0.1 mM MES pH 6.8
2.5 mM EGTA
1 mM MgCl2
Lysis Buffer: (for 20 mL)
2 mL 10X Wash Buffer (for protein samples)
10 uL of 20% Triton X-100
2 uL of 5 mg/mL Leupeptin
20 uL of 0.1 M PMSF (in 100% EtOH-stored at 4˚C)
2 uL of 1.4 mg/mL pepstatinA
2X Sample Buffer: For 10 mL
0.12 g ..... ..... .....100 mM Tris Base
0.31 g ..... ..... .....200 mM DTT
0.40 g ..... ..... .....4% SDS
tip of small spatula 0.2% Bromophenol Blue
2 mL of 100%. ..... 20% glycerol ..... .
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Clark Biology Dept | Clark University