The Larochelle Lab
at Clark University
Lab
Research goals for the upcoming academic year include:
- Continued focus on Dictyostelium as a model organism in which to study cytokinesis.
- Development of RNAi as a rapid and efficient tool for deciphering gene function in Dictyostelium.
- Using Dictyostelium to study centrosome-associated proteins and their roles in cell division.
- Genetic and molecular characterization of a novel mutant with defects in cell adhesion.
Protocols
..Protein Work...... IMMUNOPRECIPITATIONS (pdf)
...... SDS-PAGE GELS (pdf)
...... WESTERN BLOTTING (pdf)
...... SILVER STAINING
...... PROTEIN SAMPLES
...... COOMASSIE STAINING
Microscopy
.......DAPI STAINING
.......INDIRECT IMMUNOFLUORESCENCE
.......FIXING CELL
Molecular Protocols
.......
.......PCR (pdf)
.......MINIPREP (pdf)
.......
.......SEQUENCING (pdf)
.......SOUTHERN BLOT (pdf)
.......NORTHERN BLOT (pdf)
.......RNA ISOLATION
.......RNA AGAROSE GEL
.......GENOMIC DNA ISOLATION (pdf)
.......TRANSFORMATION OF E. COLI (pdf)
.......TRANSFORMATION OF E.COLI- DH5 SPECIFIC (pdf)
.......CaCl2 COMPETENT CELLS (pdf)
Cell Protocols
.......CELL CULTURE (pdf)
.......TRANSFECTION INTO DICTYOSTELIUM (pdf)
.......PASSING CELLS
.......CENTROSOME ISOLATION (pdf)
.......CENTROSOME, NUCLEI, AND ENRICHMENT-PURIFYING
Recipes Pertaining to the above protocols:
...... PROTEIN WORK (pdf)
...... MICROSCOPY (pdf)
.......MOLECULAR (pdf)
.......CELL (pdf)
.......GENERAL (pdf).
Links | About Prof Larochelle
Clark Biology Dept | Clark University