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How many fungi colonize a single grass root system?

Vandenkoornhuyse extracted total DNA from cleaned roots of the grass Arrhenatherum elatius and PCR amplified nuclear small subunit rDNA, which they cloned individually and sequenced

Phylogenetic analyses of cloned rDNAs against a dataset of diverse Fungi suggested that 49 different fungal species were present

Fungal sequences represented not only Glomales (Zygomycota; expected, because these fungi are involved in arbuscular mycorrhizal [AM] symbioses), but also Chytridiomycota, Ascomycota, and Basidiomycota, including groups that have not previously thought to include endophytes.

Several sequences appear to represent major lineages that have not been discovered previously, including some of the most basal lineages in basidiomycota and ascomycota

Potential source of error: chimeric PCR products


Vandenkoornhuyse, P., S. L. Baldauf, C. Leyval, J. Straczek, and J. P. W. Young. 2002. Extensive fungal diversity in plant roots. Science 295: 2051.

All content © 2005 AFTOL (Assembling the Fungal Tree of Life Project). Website managed by Jason Slot. AFTOL logo designed by Michal Skakuj. Contact Dr. David Hibbett with any questions. This page was last modified on 08/31/05. Development of this site is being supported by a grant from the National Science Foundation for research in fungal evolutionary biology (NSF award number DEB-0228657).